Recently, a lot of publicity focussed on stunning CT scans of embryos preserved in fossilised eggs of a Jurassic sauropodomorph dinosaur, which were obtained using very high energy X-rays generated by a synchrotron in France (Chapelle, K.E.J. et al. 2020. Conserved in-ovo cranial ossification sequences of extant saurians allow estimation of embryonic dinosaur developmental stages. Nature Scientific Reports, v. 10, article 4224; doi: 10.1038/s41598-020-60292-z). The images suggest that the embryos’ skulls developed in much the same way as do those of living reptiles. Within a week there emerged an even more compelling dinosaurian scoop: a fossil nestling of a duck-billed dinosaur (hadrosaur) from the Upper Cretaceous of Montana is reported to have yielded evidence for a broad spectrum of cellular materials (Bailleul, A.M. et al. 2020. Evidence of proteins, chromosomes and chemical markers of DNA in exceptionally preserved dinosaur cartilage. National Science Review, v. 7, advance publication NWZ206; DOI: 10.1093/nsr/nwz206).
Alida Bailleul, who works at the Chinese Academy of Sciences in Beijing, and fellow molecular palaeontologists from Canada, the US and Sweden, examined material from the nestling’s skull that was suspected to contain traces of cartilage. Their methods involved microscopic studies of thin sections together with staining and fluorochemical analysis of cellular material extracted by dissolving away bone tissue in acid. The same methodologies were also applied to similar material from modern emu chicks as a means of validating the results from the fossil. Staining used the same chemical that previously had revealed blood proteins in a specimen of Tyrannosaurus rex (see: Blood of the dinosaurs in Palaeobiology, January 2011). The fluorescence approach dosed the dinosaur cartilage with antibodies against bird collagen, and revealed an immune reaction (green fluorescence) in both fossil material and that from the baby emus.
The researchers also isolated cartilage cells (chondrocytes) from the dinosaur preparations. Two stains (PI and DAPI, for short) that show up DNA were applied, giving positive responses. The PI (propidium iodide) stain is useful as it does not respond to DNA in living material, bit only to that in dead cells, thereby helping to rule out contamination with modern material. Apparently, the double-staining experiments support the presence of double-stranded material that involves at least six base pairs (of ACTG amino acids). This does not prove the existence of dinosaur DNA, but does demonstrate that the hadrosaur’s cell nuclei are preserved.
Does that suggest that the hunt is on for a dinosaur genome, with all its connotations? OK, a complete genome has been extracted from a frozen Siberian mammoth a few tens of thousand years old, which encourages ‘re-wilding’ aficionados, but that animal preserved intact cells of many kinds. A 70 Ma old dinosaur fossil, however exquisitely preserved, is mostly ‘rock’, in that preservation is through mineralisation of bone and tissue, and even cells … Moreover, it is possible that what the team found may even be material from post-mortem bacterial colonisation of any age younger than 70 Ma.
See also: De Lazaro, E. 2020. Scientists Use X-rays to Peer inside Fossilized Dinosaur Eggs Sci News, 10 April 2020; Black, R. 2020. Possible dinosaur DNA has been found. Scientific American, 17 April 2020