Denisova Cave was named to commemorate an 18th century hermit called Denis, who used it as his refuge. The culmination of more than four decades of excavation, which followed the discovery there of Mousterian and Levallois tools there, has been the explosion onto the palaeoanthropological scene of Denisovan genomics, beginning in 2010 with sequenced DNA from a child’s finger bone. The same layer yielded Neanderthal DNA from a toe bone in 2013. Another layer yielded similar evidence in 2018 of an individual who had a Neanderthal father and a Denisovan mother. Application of the new technique of peptide mass fingerprinting, or zooarchaeology by mass spectrometry (ZooMS), to small, unidentifiable bone fragments from the cave sediments revealed further signs of Denisovan occupation and the first trace of anatomically modern humans (AMH). So far the tally is 4 Denisovans (two female children and two adult males), a Neanderthal woman and the astonishing hybrid. Analyses of the sediments themselves showed traces of both Neanderthal and Denisovan mtDNA from deeper in the stratigraphy than levels in which human fossils had been found, but which contained artefacts. The discovery of the first Denisovan DNA revealed that AMH migrants from Africa who reached the West Pacific islands about 65 ka ago carried fragments of that genome. As well as hybridising with Neanderthals some of the people who left Africa had interbred with Denisovans sufficiently often for genetic traces to have survived. Yet, until now, the ages of the analysed samples from the cave remained unknown.
That is no surprise for two reasons: cave sediments are complex, having been reworked over millennia to scramble their true stratigraphy; most of the organic remains defied 14C dating, being older than its maximum limit of determination. However, using alternative approaches has resulted in two papers in the latest issue of Nature. The first reports results from two methods that rely on the luminescence of grains of quartz and feldspar when stimulated, which measures the time since they were last exposed to light (Jacobs, Z. and 10 others 2019. Timing of archaic hominin occupation of Denisova Cave in southern Siberia. Nature, v. 565, p. 594-599; DOI: 0.1038/s41586-018-0843-2). Over 280 thousand grains in 103 sediment samples from different depths and various parts of the cave system have yielded a range of ages from 300 to 20 ka that span 3 glacial-interglacial cycles except for a few gaps, giving rough estimates of the timing of hominin occupation shown by fossils and soil layers that contain DNA. The youngest evidence for Denisovans is shown to be roughly 50 ka; a time when AMH was present elsewhere in Siberia. They lived at a time halfway between the 130 ka interglacial and the last glacial maximum. Two Neanderthals, a Denisovan and the hybrid occupied the site during the 130 ka interglacial. Soils from the previous warm episode from 250 to 200 ka contain both Neanderthal and Denisovan DNA traces. The oldest occupancy, marked by the presence of a Denisovan bone sample, was 300 ka ago, once again midway between an interglacial and a glacial maximum.
The second paper (Douka, K. and 21 others 2019. Age estimates for hominin fossils and the onset of the Upper Palaeolithic at Denisova Cave. Nature, v. 565, p. 640–644; DOI: 10.1038/s41586-018-0870-z) focused on direct dating of the hominin fossils themselves – and thus their DNA content, important in trying to piece together timings of genetic mixing. In the absence of radiocarbon dates from the bones themselves because of most specimens’ >50 ka ages, except in the case of the youngest whose 14C age is at the 50 ka limit. They resorted to a hybrid technique based on a means of modelling fossils’ ages from differences in mtDNA between the specimens and that in the youngest hominin, which, luckily, was dateable by radiocarbon means. Weighted by dating of the actual sediments that contain them, the differences should become greater for successively older fossils because of random mutations: a variant of the ‘molecular clock’ approach. It’s complicated and depends on assuming that mitochondrial mutation rate was the same as that in modern humans. Unsurprisingly the results are imprecise, but sufficient to match the hominin fossil occurrences with different environmental conditions
Pollen grains and vertebrate fossils from various levels in the cave system demonstrate the wide climatic and ecological conditions in which the various hominins lived. The warmest episodes supported broad-leafed forest, offering maximum resources for hominin survival. Those between interglacial and full glacial conditions were much less benign, with alternating dry and wet cold conditions that supported open steppe ecosystems. The oldest Denisovan occupation was at the close of a period of moderately warm and humid conditions that supported mixed conifer and broad-leafed trees that gave way to reduced tree cover.
As well as the presence of stone tools sporadically through the sedimentary sequence, in the youngest levels there are bone rings and pendants made from deer teeth; clearly ornamental items. Did the late Denisovans make them or do they signify anatomically modern human activity? Radiocarbon ages do not give a concrete answer, one of the pendants is about 45 ka old with an error that puts it just within the range of age variation of the oldest Denisovan fossil. No AMH remains have been found in Denisova Cave, but remains of a modern human male have been found at Ust’-Ishim, in NW Siberia. At 45 ka, he represents the earliest arrival of AMH in northern Asia. So it may have been members of this new population that left ornaments in Denisova, but, for the moment, artistic Denisovans are a possibility.
Further deployment of rapid screening for hominin bone fragments using the ZooMS method and analyses for traces of DNA in soils is likely to expand the geographic and time ranges of Denisovans and other close human relatives. Denisova Cave formed in Silurian limestones of the Altai Range, and there are other caves in those hills …
Related article: Dennel, R. 2019. Dating of hominin discoveries at Denisova. Nature, v. 565, p. 571-572; DOI: 10.1038/d41586-019-00264-0)
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